Buried Alive: Ecology of the Infaunal Sponge Oceanapia
Student: Mylene Gonzales
Major: Marine Biology
Mentor: Dr. Chris Freeman
Department: Biology
Buried Alive: Ecology of the Infaunal Sponge Oceanapia
Most sponges (Porifera) live attached to exposed surfaces on coral reefs or within crevices, but almost all of the biomass of sponges in the genus Oceanapia is under the sediment, with only tube-like fistules extending out of the sediment and into the surrounding environment. Preliminary experiments using fluorescein dye to visualize water flow showed that water enters the sponge through these fistules, travels through hollow tubes to the main body (ball) of the sponge, and is released after traveling through long excurrent tubes. Initial observations of Oceanapia spp. in Bocas del Toro, Panama also suggested the presence of cyanobacterial symbionts in the exposed fistules; to better understand the ecology of Oceanapia, we measured the abundance of cyanobacterial symbionts within different parts of this sponge (fistule, ball, and excurrent canal) by measuring the chlorophyll a concentration in each of these regions. We discovered that chlorophyll a values were high in the fistule, but about 9x and 29x lower in the excurrent canals and underground ball, respectively. These data support the presence of cyanobacterial symbionts in the exposed fistules and an absence of these symbionts in other regions of Oceanapia. Furthermore, to determine the role of these symbionts in carbon and nitrogen metabolism, we incubated Oceanapia sponges in seawater spiked with inorganic compounds enriched in 13C and 15N (NaH13CO3 and Na15NO3) and measured the 13C and 15N values of tissue from different regions of the sponge. These data suggest that Oceanapia engages in a unique and currently unexplored interaction with photosynthetic symbionts.